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Image Search Results
Journal: Marine Drugs
Article Title: Physicochemical and Biocompatibility Properties of Type I Collagen from the Skin of Nile Tilapia ( Oreochromis niloticus ) for Biomedical Applications
doi: 10.3390/md17030137
Figure Lengend Snippet: The effect of ASC, PSC, and porcine collagen (PC) on cell proliferation. ( A ): MC3T3E1, ( B ): L929, ( C ): HUVEC. Values with * show significant differences ( p < 0.05) between groups, as determined by one-way ANOVA.
Article Snippet: MC3T3E1 and
Techniques:
Journal: PeerJ
Article Title: miR-452-3p inhibited osteoblast differentiation by targeting Smad4
doi: 10.7717/peerj.12228
Figure Lengend Snippet: (A) The cell viability of MC3T3-E1 after 200 ng/mL BMP2 treatment for 0, 12, 24, 36, and 48 h was assessed using CCK-8 assay. (B) The number of calcium nodules was counted in 200 ng/mL BMP2-treated MC3T3-E1 cells by alizarin red staining, compared with untreated MC3T3-E1 group. (C) On days 0, 7, and 14, the mRNA levels of the osteoblast marker genes OPN, Col1A1, and Runx2 were assessed using by RT-PCR in induced MC3T3-E1 cells. (D) Alkaline phosphatase (ALP) activity in BMP2-treated MC3T3-E1 cells on days 0, 7 and 14 was measured using a commercial assay kit. (E) RT-PCR analysis of the expression of miR-452-3p in induced MC3T3-E1 cells. N = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Article Snippet: The
Techniques: CCK-8 Assay, Staining, Marker, Reverse Transcription Polymerase Chain Reaction, Activity Assay, Expressing